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. 2022 Sep 9;60(1):1710–1720. doi: 10.1080/13880209.2022.2112963

Figure 2.

Figure 2.

APS inhibited IFN-γ-induced PD-L1 expression and attenuated PD-L1-mediated immunosuppression. HCC cells including SMMC-7721 and Huh-7 cells were respectively pre-treated with 0.1 mg/mL, 0.5 mg/mL and 1 mg/mL of APS for 4 h, then APS-treated cells were treated with 10 ng/mL IFN-γ for 24 h. (A–D) The level of PD-L1 on SMMC-7721 and Huh-7 cells surface was detected by flow cytometry. (E and F) PD-L1 protein level on SMMC-7721 and Huh-7 cells surface was measured by Western blot. (G and H) Activated PBMCs cells were co-cultured with treated SMMC-7721 and Huh-7 cells at 5:1 for 72 h, SMMC-7721 and Huh-7 cells were isolated and collected. PBMCs mediated-HCC cell killing effect was detected by flow cytometry. The data were expressed as mean ± SD. All data were obtained from at least three replicate experiments (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.