Figure 4.

MSN served as a target for miR-133a-3p. SMMC-7721 and Huh-7 cells were respectively pre-treated with 0.1 mg/mL, 0.5 mg/mL and 1 mg/mL of APS for 4 h, and APS-treated cells were treated with 10 ng/mL IFN-γ for 24 h. (A) The level of MSN was assessed by qRT-PCR. miR-133a-3p inhibitor or miR-133a-3p mimic was transfected into treated SMMC-7721 and Huh-7 cells for miR-133a-3p inhibition or overexpression, NC inhibitor or mimic served as the negative control. (B and C) The level of MSN was assessed by qRT-PCR and Western blot. (D) starBase was used to predict the downstream targets of miR-133a-3p, and the direct binding relationship between miR-133a-3p and MSN was confirmed by dual-luciferase reporter assay. The data were expressed as mean ± SD. All data were obtained from at least three replicate experiments (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.