FIGURE 2.

A schematic overview from patient’s tissue sample to characterized iPSCs cultures. Patient/donor samples are chosen, the four most frequent tissues are fibroblasts, blood cells, urine track epithelial cells and keratinocytes. Reprogramming factors (RFs), enhancers, and the reprogramming strategy are carefully selected. Once iPSCs cultures are obtained, controls and characterization assays are performed as shown. Abbreviations: SeV, Sendai virus; CRISPRa, CRISPR-Cas9-based gene activation; IF, immunofluorescence; ALP, alkaline phosphatase assay; H&E, hematoxylin and eosin; SNP, single-nucleotide polymorphism; CGH, comparative genomic hybridization; CNV, copy number variation.