Skip to main content
. 2022 Sep 5;2022:7769355. doi: 10.1155/2022/7769355

Figure 5.

Figure 5

Nrf2 knockdown compromised the protective effects of QCT. (a) Representative immunoblots for PC12 cells transfected with Ctrl siRNA or Nrf2 siRNA. (b) Quantification of Nrf2 protein levels (n = 3). (c) Cell viability was assayed for transfected PC12 cells subjected to QCT (10 μM, 1 h), before being treated with MPP+ (0.5 mM, 24 h) (n = 5). (d) Representative immunoblots for SLC7A11 and GPX4 proteins from PC12 cells transfected with Nrf2 siRNA for 48 h and then treated with QCT (10 μM, 1 h) before being subjected to MPP+ (0.5 mM, 24 h). (e, f) Quantification of SLC7A11 and GPX4 protein levels (n = 3). Data are presented as the mean ± SEM. Student's t-test and one-way ANOVA followed by Tukey's post hoc test. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.