Figure 2.

Antioxidant properties of brevinin-1FL in vitro. (a) ABTS radical scavenging activity. (b) DPPH radical scavenging activity. (c) NO scavenging activity. (d) FRAP antioxidant activity. (e) Hydroxyl radical scavenging activity. Lane M: marker. Lane 1: pBR322 (0.5 μg) plasmid DNA control. pBR322 DNA was exposed to 2 mM FeSO₄ plus 30% H₂O₂ in the absence (lane 2) or the presence of brevinin-1FL (320, 160, 80, 40, and 20 μM) at 37°C for 1 h (lanes 3-7) before agarose gel electrophoresis of DNA and image capture, respectively. SC DNA: supercoiled DNA; OC DNA: open circular DNA. Data are presented as mean ± SD (n = 3). ns: no significant. ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001 compared with each concentration.