Figure 7.

Inhibition of H₂O₂-induced apoptosis and cycle arrest by brevinin-1FL in PC12 cells. PC12 cells were pretreated with different concentrations of brevinin-1FL for 30 min and then incubated with H₂O₂ for another 6 h before cell cycle and apoptosis were analyzed with flow cytometry. (a) Cell apoptosis analysis. The percentage is the ratios of normal, early, and late apoptotic and necrotic cells. (b) Cell cycle analysis. A–E in (a) and (b) are from the control group without treatment, H₂O₂ treatment group, and 2, 4, and 8 μM of brevinin-1 pretreatment groups, respectively. (c) Western blot analysis of Bax, Bcl-2, PARP, cleaved PARP, caspase 3, and cleaved caspase 3 in H₂O₂-induced PC12 cells. (d) Statistical analysis of quantified band densities of the brevinin-1FL-treated group compared with the H₂O₂-induced group. Bars represent the relative expression of the target protein to the control. Band densities were analyzed by ImageJ software, and data are mean ± SD (n = 3). ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001 compared with the H₂O₂-induced group.