FIG. 3.

LctM and truncated derivatives fused to the GAL4 activator domain. pHA888 is pGAD424 containing the insert (full-length lctM) represented by the heavy top line. The following restriction sites are indicated: B, BamHI; Bc, BclI; Bg, BglII; E, EcoRI; P, PstI. The other plasmids result from deletions in pHA888 created at the indicated restriction sites, with the deleted fragments represented by dashed lines. The encoded proteins are represented by boxes in which the domains conserved between LanM and LanC, among LanM proteins, and between class AII LanM proteins and MrsM are indicated by light shading, dark shading, and black, respectively. The domains are numbered according to Fig. 2. The numbers next to the boxes indicate their last or first residues. The β-galactosidase activities given are means ± standard errors of duplicate or triplicate assays from at least three distinct liquid cultures of yeast cells coexpressing the corresponding AD::LctM fusion and BD::LctA (pHB246).