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. 2022 Sep 8;17:4023–4038. doi: 10.2147/IJN.S377317

Figure 1.

Figure 1

The identification of SIRT1 transfection and the effects of SIRT1 knockdown and overexpression on proliferation, migration and protein expression of HSFs. (A and B) Western blot analysis of SIRT1 in HSFs transfected with mimic-control, -SIRT1 knockdown, -SIRT1 overexpression and their corresponding negative control for 48h, graph showed the relative band density to GAPDH. (C) Cell Counting Kit-8 analysis of the viability of HSFs transfected with mimic-control, -SIRT1 knockdown, -SIRT1 overexpression and their corresponding negative control at 0-, 24-, 48-, 72-, 96h. (D and E) Scratch analysis of the migration of HSFs transfected with mimic-control, -SIRT1 knockdown, -SIRT1 overexpression and their corresponding negative control at 0-, 24h, graph showed the relative closed wound area of HSFs. (F–I) Western blot analysis of NF-κB, α-SMA, and TGF-β1 in HSFs transfected with mimic-control, -SIRT1 knockdown, -SIRT1 overexpression and their corresponding negative control for 48h, graph showed the relative band density to GAPDH. Every experiment was repeated at least three times, the data was shown as mean ± SEM (**P<0.01, ***P<0.001, ****P<0.0001).

Abbreviations: SIRT1, silent information regulator 1; CON, control; NC, negative control; KD, knockdown; OE, overexpression; NF-κB, nuclear factor-kappa B; α-SMA, α-smooth muscle actin; TGF-β1, transforming growth factor-β1.