TABLE 1.

Thermal hysteresis (TH) readings for crude protein extracts from leaf tissue lysates on cold‐acclimated (CA) and non‐acclimated (NA) Bd21 wild type and temporal cold‐induced antifreeze protein knockdown lines prOmiRBdIRI‐1e and prOmiRBdIRI‐3c. Samples were tested at 40 mg ml⁻¹ of total protein concentrated from crude cell extracts. Readings were captured using a nanoliter osmometer. Assays were performed in triplicate and values shown are the average of three replicates shown with standard deviation.

Sample	TH (°C)
Buffer	0
Wild type, Bd21 (CA)	0.050 ± 0.019
Wild type, Bd21 (NA)	0.005 ± 0.003
prOmiRBdIRI‐1e (CA)	0.013 ± 0.002
prOmiRBdIRI‐1e (NA)	0.003 ± 0.003
prOmiRBdIRI‐3c (CA)	0.012 ± 0.011
prOmiRBdIRI‐3c (NA)	0.002 ± 0.002