TABLE 1.
Homodimerization of full-length proteins and protein fragments fused to the wild-type LexA DNA-binding domain (DBD) in pSR658
| LexA fusion | Lengtha | % Repressionb (mean ± SD) |
|---|---|---|
| NeuA | 419 | 78 ± 2 |
| NeuA1 | 250 (1–250) | 6 ± 2 |
| NeuB | 346 | 39 ± 6 |
| NeuC | 391 | 65 ± 5 |
| NeuD | 207 | 87 ± 1 |
| NeuD1 | 108 (1–108) | 21 ± 9 |
| NeuD2 | 118 (90–207) | 13 ± 5 |
| NeuD3 | 64 (144–207) | 16 ± 1 |
| Chloramphenicol acetyltransferase | 220 | 94 ± 2 |
a
Length of amino acid insert encoded by neu insert in pSR658. The amino acid residues of protein fragments are in parentheses.
b
Relative to pSR658 alone in strain SU101. Average β-galactosidase activity in SU101(pSR658) is 2,482 ± 254 Miller units.