Extended Data Fig. 3. The labeling coverage, but not insufficient sampling during a MINFLUX recording, limits the density of localized molecules.

The individual panels show all recorded localizations in the indicated time intervals. The full field of view of the 8-hour data set is shown in Fig. 1f. The FRC resolution was calculated using all data up to a certain time point (blue circles). After 6-7 h almost no new localizations contribute to the recorded Vimentin filament and the FRC resolution reaches a plateau, suggesting that the imaging time was sufficient to localize the vast majority of available binding sites. Scale bar: 50 nm.