FIG. 1.

Amino acid sequence comparison between the N- and C-terminal domain cupin motifs of C. velutipes oxalate decarboxylase (OxDe), those of the B. subtilis bicupins YvrK, YoaN, and YxaG, barley (Hordeum vulgare) oxalate oxidase (OxOx), and moss (Barbula unguiculata) germin-like manganese-superoxide dismutase (GLSD). The number of intervening amino acid residues between the two parts of each cupin motif is indicated. Conserved residues are highlighted with asterisks. The conserved residues in bold are predicted to ligate a Mn²⁺ ion in the active site of oxalate oxidase.