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. 2022 Sep 12;12:15313. doi: 10.1038/s41598-022-19481-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Photomicrographs illustrating the lesions resulting from HFD. (A) Fibrillation and fissuring of articular cartilage progressed to erosion of non-calcified cartilage was shown (black arrow) and the tidemark is no longer intact. Magnification × 10. (B) Most of chondrocytes were highly destructed with no clear lacunae (blue arrow) and pyknotic nuclei was also observed. Magnification × 40.