Fig. 7.

Live imaging of lectin-CAR T cells cytotoxicity. A cytotoxicity assay was performed using GFP⁺ Gb3⁺ HT-29 and the GFP⁺ Gb3⁻ LS-174 colon cancer cells and monitored by live cell imaging. a, b Data show cellular proliferation of GFP⁺ cells up to 48 h, images were taken every 3 h. c, d HT-29 and LS-174 cells were stained with C.LIVE Tox Red, a sensitive dye that binds to DNA. The dye enters dead cells through damaged plasma membranes and emits fluorescence after binding. We monitored cell fluorescence for 48 h, taking images of all wells every 3 h. Total area in green or red was quantified. Mean values ± SEM are shown. n = 4, simultaneously performed co-incubations, representative data of one experiment out of two independent donors