Fig. 2.

Abnormal subcellular localizations of mutant mini-agrin in primary motoneurons. a Representative confocal images of MNs 2 days after transfection with WT, L1, L2 or LM mini-agrin IRES eGFP (green) constructs. Overexpressed human agrins were revealed with an anti-human-agrin antibody (red). b Magnification of the cell bodies from images in a. WT agrin showed a diffuse distribution often with stronger labeling at the soma/neurite boundary characteristic of secreted proteins (arrow). L2 agrin accumulates exclusively in the soma (star). c Magnification of the axonal projections from images in a. L1 and LM agrin accumulate along the axo-dendritic compartment (white arrowhead). Scale bars: 20 μm