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. 2022 Aug 10;144(4):707–731. doi: 10.1007/s00401-022-02475-8

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Fig. 5 — Mutant FL-agrins accumulate in the endoplasmic reticulum and are less secreted. a. Confocal images of SHEP transfected cells with FL-agrin fused to eGFP (in green), counterstained with the endoplasmic reticulum marker KDEL (in red) and the Golgi apparatus marker GM130 (in white). White arrow shows agrin exclusion from the Golgi apparatus. Scale bar: 10 μm. b Quantification of agrin/Golgi co-localization (Kruskal–Wallis test followed by Dunn's multiple comparison test; **p < 0.01). c Quantification of agrin/endoplasmic reticulum (ER) co-localization (Kruskal–Wallis test followed by Dunn's multiple comparison test; *p < 0.05). d Representative dot-blot for agrin quantification in conditioned medium (CM) and whole-cell extract (WCE) from cultures expressing WT, L1, L2 or LM FL-agrins. e Graphic representation of FL-agrin secreted ratio quantified from the dot-blots and at least three independent experiments (one-way ANOVA followed by Dunnett's multiple comparison test; ***p < 0.001). c Quantification of the percentage of FL agrin that colocalized with the Golgi apparatus marker GM130 (Kruskal–Wallis test followed by Dunn's multiple comparison test; **p < 0.01)