Fig. 7.

Mutant agrins trigger apoptosis in vitro and in ovo. a Representative images of SHEP cultures co-expressing mini-agrin and eGFP (green). Blue: nuclei are stained with DAPI. Red: activated caspase-3 immunostaining. Scale bar: 200 µm. b–d Quantification of activated caspase-3 positive cells and mean cell area 48 h after transfection, and quantification of survival at 72 h (n = 3; One-way ANOVA followed by Dunnett's Multiple Comparison Test). e Representative confocal images of activated caspase-3 immunostaining (red) in electroporated neurons co-expressing mini-agrin and eGFP (green) in ovo. Scale bar: 100 µm. f Quantification of activated caspase-3 positive neurons in spinal cord cryosections spanning the entire electroporated spinal cord area (n = 6 embryos per condition from 3 independent electroporation sessions; one-way ANOVA followed by Dunnett's multiple comparison test). CL corresponds to the same quantification on the contralateral side of the spinal cord that did not receive the transgene