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. 2022 Sep 12;5:956. doi: 10.1038/s42003-022-03882-z

Table 1.

Binding affinities of soluble cyt b5 to SCD1 or soluble b5R.

KD (µM) b5R SCD1
cyt b5 (WT) 2.71 ± 0.33 4.42 ± 1.77
cyt b5 (E43A) 9.63 ± 0.97 11.2 ± 2.6
cyt b5 (E49A) 8.45 ± 1.44 8.23 ± 2.49

KD values are denoted as mean ± SEM calculated from three measurements (n = 3). Sensorgrams are shown in Supplementary Fig. 6.