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. 2022 Sep 12;13:5239. doi: 10.1038/s41467-022-32848-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The 3D whole-organ images of mouse lymphatic vessels in the lung, brain, intestine, heart, kidney, stomach, pancreas, and eye. Prox1-GFP mice (2-4 months) were sacrificed and subjected to CUBIC procedures as shown in Fig. 1a. To enhance the signals from Prox1-GFP, samples were stained with anti-GFP antibody (Z = 10 μm step, digital zoom; lung, brain, stomach, and pancreas 1.25, intestine and kidney: 1.6, heart: 2.0, eye: 4.0). The enlarged 3D images (white insets) are shown in the bottom. b The enlarged 3D images of the heart and eye shown in (a). c, d Simultaneous visualization of blood and lymphatic vessels in the stomach and intestine. VE-cad-tdTomato-Prox1-GFP mice (2-4 months) were sacrificed, and samples were subjected to CUBIC procedures. The 3D whole-stomach (c) and intestine (d) images are shown. The enlarged images of white insets are shown next to whole-organ images. The 2D images (XY) are also shown in the right panel (Z = 10 μm step, digital zoom; stomach: 1.25, intestine: 1.6 and 6.3).