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. 2022 Jun 15;38(9):1041–1056. doi: 10.1007/s12264-022-00892-z

Fig. 8.

Fig. 8

Activation of VTA-LEC projection terminals or injection of quinpirole into the superficial layer of LEC alleviates behavioral hypersensitivity induced by CFA. Aa, Expression of AAV-hM3Dq-mCherry in the VTA 28 days after viral injection (scale bar, 500 µm). Ab, Expression of mCherry-positive fibers and terminals in the LEC (scale bar, 500 µm; insert, magnified image showing mCherry-positive fibers). Ac Histological verification of drug diffusion in the LEC (scale bar, 500 µm; L1, layer 1). B, C Effects of intra-LEC injection of vehicle or CNO (3 µmol/L) in AAV-hM3Dq-mCherry- or AAV-EGFP-infected CFA rats on paw withdrawal thermal latencies (B) and mechanical thresholds (C) for paws ipsilateral to the CFA injection. ** vs AAV-EGFP + vehicle. D, E Thermal latencies (D) and mechanical thresholds (E) of AAV-hM3Dq-mCherry-infected CFA rats with or without intra-LEC pre-injection of vehicle, SCH23390 (50 µmol/L), or sulpiride (50 µmol/L) before CNO application. * vs CNO. F Effect of intra-LEC injection of quinpirole, vehicle, or 8-Br-cAMP + quinpirole on thermal latency and mechanical threshold of the left and right hindpaws (CFA was injected into the left hindpaw; L, left; R, right). G Thermal latencies and mechanical thresholds of CFA-injured paws, normalized to the non-injured paws, in rats receiving quinpirole, vehicle, or 8-Br-cAMP + quinpirole injection. *P < 0.05, **P < 0.01; n.s., not significant.