Table 2.

Kinetic parameters of PrpD, PrpD^VTL, PrpD^VT, and PrpD^VL

Enzyme	Substrate	kcat (s−1)	Km (mM)	kcat/Km (mM−1 s−1)
PrpD	2-methylcitrate	0.045 ± 0.025	0.96 ± 0.85	0.075 ± 0.054
	Citrate	10,561.95 ± 3,427.82	66.39 ± 22.03	159.37 ± 2.36
	cis-Aconitate	119.03 ± 13.42	0.70 ± 0.10	170.61 ± 5.65
PrpDVTL	2-methylcitrate	0.024 ± 0.008	1.96 ± 0.21	0.013 ± 0.005
	Citrate	9,586.41 ± 1,193.47	35.32 ± 5.17	272.17 ± 11.72
	cis-Aconitate	81.83 ± 14.14	0.72 ± 0.10	113.60 ± 14.61
PrpDVT	2-methylcitrate	0.023 ± 0.008	1.93 ± 0.21	0.012 ± 0.006
	Citrate	6,093.22 ± 1,122.92	14.82 ± 2.38	410.08 ± 22.85
	cis-Aconitate	172.08 ± 19.98	1.72 ± 0.25	100.52 ± 3.18
PrpDVL	2-methylcitrate	0.035 ± 0.009	1.72 ± 0.54	0.021 ± 0.002
	Citrate	7,804.72 ± 693.10	15.81 ± 1.89	494.97 ± 15.22
	cis-Aconitate	96.34 ± 4.55	1.52 ± 0.08	63.63 ± 3.33

Source data are provided as a Source Data file.