Table 3.

In-vitro antioxidant activity of aqueous extract of barley and wheat grass extract at concentration 0.1–1 mg/mL. All values were expressed as Mean ± SD.

Concentration	Hydrogen peroxide (H2O2●) scavenging activity	Hydroxyl radical (OH●) scavenging activity	Nitric oxide (NO●) scavenging activity	Metal chelating activity	Ferric reducing scavenging activity Absorbance (nm)	Total antioxidant activity Absorbance (nm)
Barley grass extract
0.2 mg/mL	26.723 ± 0.053	63.850 ± 0.010	42.714 ± 0.054	20.414 ± 0.014	0.085 ± 0.007	0.204 ± 0.022
0.4 mg/mL	30.441 ± 0.040	72.406 ± 0.015	51.172 ± 0.057	34.409 ± 0.027	0.148 ± 0.018	0.251 ± 0.022
0.6 mg/mL	38.917 ± 0.192	77.682 ± 0.050	60.515 ± 0.048	36.438 ± 0.053	0.233 ± 0.041	0.628 ± 0.022
0.8 mg/mL	45.569 ± 0.020	82.602 ± 0.050	68.512 ± 0.094	47.567 ± 0.018	0.483 ± 0.013	0.393 ± 0.044
1 mg/mL	58.404 ± 0.020	88.484 ± 0.024	72.049 ± 0.062	56.666 ± 0.062	0.388 ± 0.051	0.487 ± 0.088
Wheat grass extract
0.2 mg/mL	23.646 ± 0.433	57.433 ± 0.087	33.487 ± 0.302	16.501 ± 0.029	0.063 ± 0.000	0.157 ± 0.022
0.4 mg/mL	26.168 ± 1.853	66.131 ± 0.100	47.712 ± 0.054	23.674 ± 0.011	0.116 ± 0.038	0.220 ± 0.022
0.6 mg/mL	31.766 ± 0.157	70.909 ± 0.046	56.362 ± 0.043	34.606 ± 0.037	0.196 ± 0.002	0.566 ± 0.038
0.8 mg/mL	35.626 ± 0.312	75.935 ± 0.037	59.900 ± 0.072	42.380 ± 0.083	0.425 ± 0.014	0.361 ± 0.022
1 mg/mL	38.860 ± 0.179	84.456 ± 0.055	65.551 ± 0.108	51.169 ± 0.025	0.334 ± 0.075	0.455 ± 0.022