FIG. 5.

Transcriptional analysis of ssgA. (A) Analysis of samples taken from normal growth curve in MM. Lanes 1 to 5 refer to samples taken at an OD₅₅₀ of 0.3 (early log), 0.6 (mid-log), 0.9 (late log), 1.2 (transition phase), and 1.5 (stationary phase), respectively. (B) Analysis of samples taken after nutritional shift-down. Lanes 1 to 6 refer to samples taken 0, 15, 30, 60, 120, and 240 min after shift-down, respectively. Lane M, ³²P-end-labeled HaeIII-digested ϕX174 size markers; numbers on the right refer to sizes of the marker bands (in nucleotides). Positions of the ssgA transcript and reannealed probe are shown on the left.