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In vitro knockdown of poldip2 inhibited oxidative stress. (A–F) The expression of poldip2, Nox4, VCAM-1, HIF-1α and VEGF in HRMECs is demonstrated in different treatments. (G–I) Levels of ROS and H2O2 in HRMECs in different siRNA treatments. Student's t-test. Results are from three independent replicate experiments, data represents mean ± SEM, n = 3/group, scale bar: 50 μm. Compared with si-NC group: **p < 0.01. si-NC: control group, si-poldip2: poldip2 siRNA transfection group.
