Table 1.
Contains information regarding the catalogue number of all reagents, cell lines, and kits used.
| categories | vendor | catalog number | dilution ratio |
|---|---|---|---|
| Primary antibodies | |||
| TRPV1 | abcam | ab6166 | 1:1000 (WB) |
| 1:100 (IHC) | |||
| PPARγ | santa cruz | sc-7237 | 1:200 (WB) |
| p-PPARγ | affinity | AF3284 | 1:500 (WB) |
| Poldip2 | abcam | ab181841 | 1:1000 (WB) |
| 1:100 (IHC) | |||
| ubiquitin | proteintech | 10201-2-AP | 1:1000 (WB) |
| Nox4 | novus | NB110-58849 | 1:1000 (WB) |
| 1:100 (IHC) | |||
| HIF-1α | abcam | ab1 | 1:500 (WB) |
| VCAM-1 | abcam | ab134047 | 1:2000 (WB) |
| VEGF | abcam | ab32152 | 1:1000 (WB) |
| actin | sigma | A5441 | 1:5000 (WB) |
| Secondary antibodies | |||
| goat anti-mouse IgG | ZSGB-BIO | ZB-2301 | 1:5000 (WB) |
| goat anti-rabbit IgG |
ZSGB-BIO |
ZB-2305 |
1:5000 (WB) |
|
reagents |
vendor |
Catalog number |
|
| Total cholesterol assay kit | NanJing JianCheng | A111-1-1 | |
| Triglyceride assay kit | NanJing JianCheng | A110-1-1 | |
| High-density lipoprotein cholesterol assay kit | NanJing JianCheng | A113-1-1 | |
| Low-density lipoprotein cholesterol assay kit | NanJing JianCheng | A112-1-1 | |
| BCA protein assay kit | Beyotime | P0010 | |
| ROS assay kit | Sigma | D7008 | |
| Reactive oxygen species assay kit | Beyotime | S0033S | |
| Hydrogen peroxide assay kit | Beyotime | S0038 | |
| Hematoxylin-Eosin kit | Solarbio | G1120 | |
| Cell Counting Kit-8 (CCK-8) | Targetmol | C0005 | |
| Capsaicin | Sigma | 15971 | |
| Streptozotocin | Sigma | S0130 | |
| Evans Blue | Sigma | E2129 | |
| RIPA Lysis Buffer | Beyotime | P0013B | |
| Phenylmethanesulfonyl fluoride | Beyotime | ST505 | |
| Phosphatase inhibitor cocktail | Beyotime | P1082 | |
| PVDF membrane | Millipore | ISEQ00010 | |
| Evans blue dye | Sigma-Aldrich | E2129 | |
| Matrigel Matrix | Corning | 356234 | |
| FAS eye fixative solution | Servicebio | G1109 | |
| Antifade mounting medium | Beyotime | P0131 | |
| HRMECs | Innoprot | P10880 | |
| Endothelial cell medium | ScienCell | 1001 | |
| Fetal bovine serum (FBS) | Gibco | 10099141C | |
| Penicillin-streptomycin | Beyotime | C0222 | |