TABLE 3.
Nutrient and fermentation quality of total mixed ration (TMR) after 60 days of fermentation.
| Group | ||||||
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| Initial value | I | II | III | VI | V | |
| pH | 6.78 ± 0.02a | 4.51 ± 0.02b | 4.09 ± 0.07c | 4.10 ± 0.06c | 4.05 ± 0.17c | 4.23 ± 0.09c |
| DM (%FM) | 43.10 ± 0.02a | 38.23 ± 0.68c | 39.51 ± 1.74b | 38.44 ± 2.05c | 39.69 ± 1.83b | 38.47 ± 1.07c |
| CP (%DM) | 15.28 ± 1.57 | 14.18 ± 2.33 | 14.86 ± 1.93 | 15.09 ± 2.05 | 15.13 ± 2.12 | 14.73 ± 1.97 |
| WSC (%DM) | 16.15 ± 0.89a | 7.53 ± 0.87b | 6.59 ± 0.91d | 6.86 ± 0.45c | 6.65 ± 0.43d | 7.05 ± 0.67c |
| AN (%TN) | – | 3.32 ± 0.77 | 3.24 ± 0.23 | 3.27 ± 0.59 | 3.16 ± 0.65 | 3.30 ± 0.43 |
| LAB (lg CFU/g) | 4.16 ± 0.15d | 7.37 ± 0.16c | 8.16 ± 0.29b | 8.21 ± 0.21b | 8.52 ± 0.15a | 7.93 ± 0.18b |
| Yeast (lg CFU/g) | 6.40 ± 0.32a | 3.21 ± 0.06b | 3.12 ± 0.15b | 3.15 ± 0.08b | 2.98 ± 0.19b | 3.19 ± 0.15b |
| Aerobic Bacteria (lg CFU/g) | 7.21 ± 0.29a | 3.54 ± 0.18b | 1.70 ± 0.05c | 1.74 ± 0.05c | 1.59 ± 0.06c | 1.82 ± 0.11c |
| Mold (g CFU/g) | 6.11 ± 0.10a | 1.67 ± 0.58b | ND | ND | ND | 1.00 ± 0.02b |
Different lowercase letters indicate significant differences between peer data (P < 0.05), ND, not detected; DM, dry matter; CP, crude protein; WSC, water soluble carbohydrates; AN, ammonia nitrogen.