TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant charcteristic(s) | Source or reference |
|---|---|---|
| E. coli DH5α MCR | F−mcrAΔ1 (mrr-hsd RMS-mcrBC) Φ80dlacZ Δ(lacZYA-argF)U169 deoR recA1 endA1 supE44 λ thi-1 gyr-496 relA1 | Gibco-BRL |
| B. subtilis | ||
| BD99 (wild type) | hisA1 thrS trpC2 | A. Garro |
| VK1A | BD99 ΔmrpA | 9 |
| VK1B | BD99 ΔmrpB | This study |
| VK1C | BD99 ΔmrpC | This study |
| VK1D | BD99 ΔmrpD::Spr | This study |
| VK1E | BD99 ΔmrpE | This study |
| VK1F | BD99 ΔmrpF::Spr | This study |
| VK1G | BD99 ΔmrpG::Spr | This study |
| VKN1 | BD99 ΔmrpA-G::Spr | 9 |
| VK6 | BD99 mrpA::Spr | This study |
| Plasmids | ||
| pGEM11Zf(+) | Cloning vector (Apr) | Promega |
| pDH88 | Cmr vector for cloning B. subtilis chromosomal DNA and integration into the corresponding locus | 5 |
| pDR67 | amyE integration vector with Cmr gene and pspac promoter upstream of multiple cloning site | 8 |
| pDHB1 | pDH88 + ΔmrpB | This study |
| pDHC1 | pDH88 + ΔmrpC | This study |
| pDHE1 | pDH88 + ΔmrpE | This study |
| pDRB1 | pDR67 + mrpB | This study |
| pDRC1 | pDR67 + mrpC | This study |
| pDRD1 | pDR67 + mrpD | This study |
| pDRE1 | pDR67 + mrpE | This study |
| pDRF1 | pDR67 + mrpF | This study |
| pDRG1 | pDR67 + mrpG | This study |
| pGEMD1 | pGEM11Zf(+) + ΔmrpD::Spr | This study |
| pGEMF1 | pGEM11Zf(+) + ΔmrpF::Spr | This study |
| pGEMG1 | pGEM11Zf(+) + ΔmrpG::Spr | This study |