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. 2022 Aug 30;10:889203. doi: 10.3389/fchem.2022.889203

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Copyright © 2022 Xue, Zhang, Liu, Wang and Ren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Missing-wedge–corrected 3D reconstruction of DNA origami double-layered (DL) lattice. (A) A cryo-ET image of a 2D DL lattice of DNA origami octahedral cage with ferritin protein imbedded in an extended sheet of vitreous ice. (B) Representative cryo-ET images showing the two ferritins in the top and bottom layers at tilted angles (black and white arrows) in a DL lattice. (C) Selected lattice area of cryo-ET 3D reconstruction (without averaging). (D) Representative 3D density maps after missing-wedge correction by LoTToR. Reproduced with permission (Wang et al., 2021b).