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. 2022 Aug 30;10:889203. doi: 10.3389/fchem.2022.889203

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Copyright © 2022 Xue, Zhang, Liu, Wang and Ren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Missing-wedge correction on simulated 3D maps of reconstructed GroEL by LoTToR. (A) Two perpendicular views of the original object, a GroEL particle. (B and C) Projection and its Fourier transform of the 3D map along the X–Z plane. (D) Two perpendicular views of the initial 3D map, which was reconstructed from a noisy tilt series (SNR = 0.3) within a tilt angle range of ±15^o in steps of 1.5^o. (E and F) Its corresponding projection and Fourier transform of the initial 3D map along the X–Z plane. (G) Final 3D map after missing-wedge correction (after 1,000 iterations), is shown from two perpendicular views. (H and I) Corresponding projection and Fourier transform of the final 3D map along the X–Z plane. Scale bars: 50 nm. Reproduced with permission (Zhai et al., 2020).