FIGURE 4.

shRNA-mediated Nrf2 knockdown diminished the protective effects of RES and FGF1 co-treatment against DOX-induced oxidative stress in primary hepatocytes. (A-C) Relative protein expression levels of n-NRF2 and HO-1 in primary hepatocytes after the indicated treatments. Three independent experiments were performed. β-actin or histone H3 was used as the loading control. (D,E) Representative fluorescence microscopy images of DHE-stained primary hepatocytes after the indicated treatments (n = 3). (F–I) Relative mRNA expression levels of oxidative stress markers Cat, Sod, Ho-1, and Nqo1 in primary hepatocytes after the indicated treatments (n = 3). Data are expressed as mean ± SD. *p < 0.05; ns indicates not significant.