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Polymerase chain reaction conditions for amplifying blaOXA
| Stage | Amplification | Temperature (℃) | Time (s) | No. of cycles |
|---|---|---|---|---|
| Hot start | Pre-denaturation | 94 | 300 (blaOXA) | 1 |
| Cycle | Denaturation | 94 | 25 | 30 (blaOXA) |
| Annealing | 52 | 40 | ||
| Extension | 72 | 50 | ||
| Final extension | 72 | 360 | 1 |
