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Hydrolysis of cellooligosaccharides by CelZ and CelY. Each test contained approximately 0.07 IU of CMCase per ml (2-h incubation, 35°C). Abbreviations: S, mixed-cellooligosaccharide standard; G1, glucose; G2, cellobiose; G3, cellotriose; G4, cellotetraose; and G5, cellopentaose. (A) Before hydrolysis; (B) after incubation with CelY; (C) after incubation with CelZ; (D) CelZ hydrolysis of cellopentaose after different periods of incubation (0, 5, 10, and 25 min).
