TABLE 2.
Effects of E. chrysanthemi out genes on the expression and secretion of celY and celZ in E. coli DH5α
| Enzyme expressed | Promoter | Growth (h) | Without out genes
|
With out genes (+ pCCP2006)
|
||||
|---|---|---|---|---|---|---|---|---|
| Extracellular CMCasea (IU per liter) | Total CMCase (IU per liter) | Apparent secretion (%) | Extracellular CMCase (IU per liter) | Total CMCase (IU per liter) | Apparent secretion (%) | |||
| CelY | Native promoter (pMH18) | 24 | 136 | 165 | 82 | 136 | 180 | 76 |
| lac promoter (pLOI2311) | 8 | 208 | 266 | 78 | NDb | ND | ND | |
| 16 | 1,420 | 1,590 | 90 | ND | ND | ND | ||
| 24 | 1,650 | 1,800 | 90 | 1,360 | 1,510 | 90 | ||
| CelZ | Native plus lac promoter (pLOI1620) | 8 | 130 | 1,320 | 10 | 6,710 | 7,460 | 90 |
| 16 | 1,200 | 9,030 | 13 | 13,400 | 19,700 | 68 | ||
| 24 | 1,800 | 12,500 | 14 | 23,600 | 36,800 | 64 | ||
a
CMCase activity secreted or released in the culture supernatant.
b
ND, not determined.