TABLE 3.

Effect of substrate concentration on synergy

Amt of CMC substrate (g/liter)	Concn of reducing sugar released (μmol/ml)a			Avg synergyb ± SD
	CelZ	CelY	CelZ + CelY
20	3.98 ± 0.04	3.83 ± 0.04	7.51 ± 0.07	1.89 ± 0.02
10	4.53 ± 0.01	2.91 ± 0.07	5.38 ± 0.04	1.25 ± 0.01
5.0	2.87 ± 0.01	1.18 ± 0.04	2.92 ± 0.04	1.08 ± 0.02
2.5	1.42 ± 0.01	0.50 ± 0.04	1.49 ± 0.01	1.12 ± 0.01

^aAverages ± standard deviations. CelZ and CelY were diluted to equal CMCase activities. Reaction mixtures (0.15 IU/ml) with both CelZ and CelY contained 9 parts CelZ and 1 part CelY. As controls, CelZ (0.15 IU/ml) and CelY (0.15 IU/ml) were each tested individually. 

^bSynergy was calculated as the observed activity divided by the sum of predicted contributions from CelY alone (10%) plus CelZ alone (90%).