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. 2022 Aug 30;13:971732. doi: 10.3389/fphys.2022.971732

FIGURE 5.

FIGURE 5

Effects of uc.375 overexpression and FoxA1 overexpression on the function of MLE 12 cells. (A) The proliferation activity of MLE 12 cells was detected by CCK-8 assay. (B) The cell apoptosis was determined by Hoechst/PI staining in MLE 12 cells. (C) The protein levels of caspase3, FoxA1, SP-C and UCP2 were investigated by western blot in MLE 12 cells. (D) The cell apoptosis was determined using low cytometry assay in MLE 12 cells. (E-H) The relative mRNA expression levels of uc.375, FoxA1, SP-C and UCP2 were detected by qRT-PCR in MLE 12 cells. *p < 0.05, **p < 0.01 vs control. #p < 0.05, ##p < 0.01 vs uc.375 OEX.