PCR from genomic DNA using different HIP primers. HIP primers were used for PCR with 5 ng of genomic DNA template. Primers used were HIPA (lane 2), HIPC (lane 3), W2-HIPA (lane 4), W2-HIPC (lane 5), W2-HIPAC (lane 6), and HIPACC (lane 7). Molecular weight markers, in kilobase pairs, are shown in lane 1. PCR conditions are described in Materials and Methods.