FIG. 5.

Map and expression of the rpl11-rpl1 operon. (A) The map of the rpl11-rpl1 operon shows the relative positions and sizes of nusG, rpl11, and rpl1. The positions of the W2-HIPG elements are shown as black rectangles below the map. The thin line below the map represents the size and position of the differentially displayed product. (B) Probes specific for rpl11 and rpl1 were made as described in Materials and Methods and used for RPA. In the RPA for rpl11 (top), the undigested probe migrates at 162 bp and is indicated with a filled arrow. A strongly protected fragment of 102 bp, indicated by an open arrow, is seen in lane 2 (HL), with a much fainter protected fragment in lane 1 (LL). Similar results are observed in the lower panel, in which a rpl1-specific probe was used (sizes of the unprotected and protected fragments are 304 and 223 bp, respectively). Lanes 3 in both panels show the control (C) (total RNA replaced with yeast RNA), in which no protected fragment is seen.