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Mitochondrial and cytoplasmic Ca2+ recordings in the enamel cell line LS8 cells. Live-cell imaging using epifluorescence inverted microscopy of cultured LS8 cells loaded with cytoplasmic (Fura 2-AM) and mitochondrial (Rhod-2) fluorescent probes. Plot shows increase in cytosolic and mitochondrial Ca2+ after addition of thaspigargin (1.5 μM) in 0 Ca2+ and following 2mM Ca2+ restitution.
