Table 2.
Multiple reaction monitoring transitions and mass spectrometry settings for the CYP probes, aflatoxin B1 (AFB1) and internal standards used.
| Channel | Retention time (min) | Precursor ion (Da) | Product ion (Da) | Dwell (s) | Cone (V) | Collision (eV) |
|---|---|---|---|---|---|---|
| Midazolam | ||||||
| 1 | 5.16 | 326.0 | 223.0 | 0.033 | 50.00 | 30.00 |
| 2 | 5.16 | 326.0 | 249.0 | 0.033 | 50.00 | 30.00 |
| 3 | 5.16 | 326.0 | 291.0 | 0.033 | 50.00 | 20.00 |
| α-OH-Midazolam | ||||||
| 1 | 6.95 | 342.0 | 203.0 | 0.033 | 25.00 | 22.00 |
| 2 | 6.95 | 342.0 | 289.0 | 0.033 | 25.00 | 22.00 |
| 3 | 6.95 | 342.0 | 324.0 | 0.033 | 25.00 | 20.00 |
| Phenacetin | ||||||
| 1 | 3.32 | 180.1 | 110.0 | 0.026 | 40.00 | 13.00 |
| 2 | 3.32 | 180.1 | 138.0 | 0.026 | 40.00 | 10.00 |
| 3 | 3.32 | 180.1 | 152.0 | 0.026 | 40.00 | 10.00 |
| Acetaminophen | ||||||
| 1 | 1.23 | 152.1 | 93.00 | 0.108 | 40.00 | 20.00 |
| 2 | 1.23 | 152.1 | 110.0 | 0.108 | 40.00 | 10.00 |
| 3 | 1.23 | 152.1 | 134.0 | 0.108 | 40.00 | 8.00 |
| Chlorpropamide (IS) | ||||||
| 1 | 6.33 | 277.0 | 111.0 | 0.033 | 30.00 | 25.00 |
| 2 | 6.33 | 277.0 | 175.0 | 0.033 | 30.00 | 12.00 |
| 3 | 6.33 | 277.0 | 192.0 | 0.033 | 30.00 | 7.00 |
| Aflatoxin B1 | ||||||
| 1 | 5.07 | 313.0 | 241.1 | 0.033 | 65.00 | 32.00 |
| 2 | 5.07 | 313.0 | 270.1 | 0.033 | 70.00 | 35.00 |
| Zearalanon (IS) | ||||||
| 1 | 9.13 | 321.0 | 189.1 | 0.033 | 35.00 | 22.00 |
| 2 | 9.13 | 321.0 | 303.3 | 0.033 | 35.00 | 14.00 |
IS, internal standard.