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Viability of primary ovarian cells (POCs) in the 3D scaffold in vitro. (A) The isolated POCs included a vast majority of stromal cells (POCs-1) and a large number of follicles at different developmental stages (POCs-2 and POCs-3). (B) Using the live/dead assay to examine the POCs survival in the 3D scaffolds in vitro. Green fluorescence refers to living cells containing calcein AM, while red fluorescence refers to dead cells containing EthD-1.
