3D bioprinting of chondrocytes, cardiomyocytes, hepatocytes, and adipose-derived stem cells (ASCs) into living tissues/organs using a pioneered 3D bioprinter made at Prof. Wang’s laboratory in Tsinghua University: (A) The pioneered 3D bioprinter, (B) schematic description of a cell-laden gelatin-based hydrogel being printed into a grid lattice using the 3D bioprinter, (C) schematic description of the cell-laden gelatin-based hydrogel being printed into large scale-up 3D construct using the 3D bioprinter, (D) 3D printing process of a chondrocyte-laden gelatin-based construct, (E) a grid 3D construct made from a cardiomyocyte-laden gelatin-based hydrogel, (F) hepatocytes encapsulated in a gelatin-based hydrogel after 3D printing, (G) hepatocytes in a gelatin-based hydrogel after 3D printing, (H) a gelatin-based hydrogel after 3D printing, (I-P) hepatocytes in some gelatin-based hydrogels after certain periods of in vitro cultures. Reprinted from, Cryobiology, Vol 61 Issue 3, Wang X, Xu H, Incorporation of DMSO and dextran-40 into a gelatin/alginate hydrogel for controlled assembled cell cryopreservation, 345-351., Copyright (2010), with permission from Elsevier.