KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-GFP | Roche | Cat # 11814460001; RRID: AB_390913 |
| Mouse anti-Pgk1 | Molecular Probes | Cat # 22C5D8; RRID: AB_2532235 |
| Bacterial and virus strains | ||
| Rosetta2, E. coli | Novagen | Cat # 71400 |
| DH5α, E. coli | New England Biolabs | Cat # C2987I |
| Chemicals, peptides, and recombinant proteins | ||
| GTP | Thermo Fisher | Cat # R0461 |
| Calf intestinal alkaline phosphatase | Sigma | Cat # P4978 |
| 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) | Avanti Polar Lipids | Cat # 850375 |
| 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt) (Nickel-DOGS) | Avanti Polar Lipids | Cat # 790404 |
| 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotricarbocyanine Iodide (DiR’; DilC18(7)) | Thermo Fisher | Cat# D12731 |
| Fos-choline-8, fluorinated | Anatrace | Cat # F300F |
| Deposited data | ||
| ΔN17-Arf1-GDP crystal structure | Renault et al. (2003) | PDB: 1R8S |
| Myristoylated-Arf1-GTP NMR structure | Liu et al. (2010) | PDB: 2KSQ |
| Gea2-Arf1 complex model | This paper | PDB:7URO |
| closed/open model | This paper | PDB:7URR |
| closed/closed model | This paper | PDB:7UT4 |
| open/open model | This paper | PDB:7UTH |
| Gea2-Arf1 complex composite map | This paper | EMD-26716 |
| closed/open composite map | This paper | EMD-26717 |
| closed/closed composite map | This paper | EMD-26754 |
| open/open composite map | This paper | EMD-26770 |
| Gea2-Arf1 complex consensus map | This paper | EMD-26749 |
| Gea2-Arf1 complex DCB-HUS domains focused map | This paper | EMD-26750 |
| Gea2-Arf1 complex GEF domain-Arf1 focused map | This paper | EMD-26751 |
| Gea2-Arf1 complex HDS domains focused map | This paper | EMD-26752 |
| Gea2-Arf1 complex dimer interface focused map | This paper | EMD-26753 |
| closed/closed consensus map | This paper | EMD-26755 |
| closed/closed DCB-HUS domains focused map | This paper | EMD-26765 |
| closed/closed GEF domain focused map | This paper | EMD-26766 |
| closed/closed HDS domains focused map | This paper | EMD-26769 |
| closed/closed dimer interface focused map | This paper | EMD-26777 |
| open/open consensus map | This paper | EMD-26771 |
| open/open DCB-HUS domains focused map | This paper | EMD-26773 |
| open/open GEF domain focused map | This paper | EMD-26774 |
| open/open HDS domains focused map | This paper | EMD-26775 |
| open/open dimer interface focused map | This paper | EMD-26776 |
| closed/open consensus map | This paper | EMD-26797 |
| closed/open closed monomer DCB-HUS domains focused map | This paper | EMD-26779 |
| closed/open closed monomer GEF domain focused map | This paper | EMD-26780 |
| closed/open closed monomer HDS domains focused map | This paper | EMD-26781 |
| closed/open open monomer DCB-HUS domains focused map | This paper | EMD-26783 |
| closed/open open monomer GEF domain focused map | This paper | EMD-26784 |
| closed/open open monomer HDS domains focused map | This paper | EMD-26785 |
| closed/open dimer interface focused map | This paper | EMD-26778 |
| Experimental models: Organisms/strains | ||
| MATα suc2-Δ9 ura3-52 his3-Δ200 leu2-3,112 lys2-801 trp1-Δ901 | Robinson et al. (1988) | SEY6210 |
| BY4741α gea1Δ::KanMX gea2Δ::HIS3 +pCF1248 | Gustafson and Fromme (2017) | CFY2872 |
| SEY6210 gea2Δ::KanMX | This study | CFY1470 |
| KM71H (P. pastoris) | ThermoFisher Scientific | Cat # C18200 |
| KM71H pAOX1::6xHis-TEV-Gea2::BleoR | This paper | CFY3882 |
| KM71H pAOX1::6xHis-TEV-Gea2 (Y1001D)::BleoR | This paper | CFY4619 |
| Recombinant DNA | ||
| pPICZ: P. pastoris integration plasmid | ThermoFisher Scientific | Cat # V19020 |
| pRS416-GEA2 | Gustafson and Fromme (2017) | pCF1248 |
| pRS415-Gea2-GFP | Gustafson and Fromme (2017) | pMG001 |
| pRS415-Gea2(Δ996-1004)-GFP | This paper | pAM043 |
| pRS415-Gea2(Y1001D)-GFP | This paper | pAM045 |
| pPICZ-6xHis-TEV-Gea2 | This paper | pAM034 |
| pPICZ-6xHis-TEV-Gea2(Y1001D) | This paper | pAM050 |
| pCF1053: ΔN17-Arf1 | Richardson et al. (2012), | pCF1053 |
| expression plasmid | Richardson and Fromme (2015) | |
| Full-length Arf1 expression plasmid | Weiss et al. (1989) | pArf1 |
| Nmt1 expression | Duronio et al. (1990) | pNmt1 |
| Software and algorithms | ||
| SerialEM | Mastronarde (2005) | https://bio3d.colorado.edu/SerialEM/ |
| MotionCor2 | Zheng et al. (2017) | https://emcore.ucsf.edu/ucsf-software |
| CryoSPARC | Punjani et al. (2017) | https://cryosparc.com |
| RELION | Zivanov et al. (2018), Zivanov et al. (2020) | https://relion.readthedocs.io/en/release-3.1/ |
| Phenix | Liebschner et al. (2019) | https://www.phenix-online.org/documentation/index.html |
| Coot | Emsley et al. (2010) | http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot |
| TOPAZ | Bepler et al. (2019), Bepler et al. (2020) | https://cb.csail.mit.edu/cb/topaz/ |
| SBGRID | Morin et al., 2013 | https://sbgrid.org |
| Prism | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| FIJI/ImageJ | Schindelin et al. (2012) | https://imagej.net/Fiji |
| Slidebook | Intelligent Imaging Innovations | https://www.intelligent-imaging.com/slidebook |
| Other | ||
| Holey Carbon Grids, R 1.2/1.3, Au 300 mesh | Quantifoil | Cat # N1-C14nAu30-01 |
| MonoQ 5/50 GL | GE Healthcare | Cat # 17516601 |
| Ni-NTA Agarose Resin | Qiagen | Cat # 30210 |
| Superdex 200 Increase 10/300 | GE Healthcare | Cat # 28990944 |