TABLE 4.
agr polymorphism among S. aureus strains isolated from both persistent and intermittent nasal carriersa
| Person no. | Strain no. | Date of isolation (day-mo-yr) | agr type | agr group | RAPD type | No. of protA repeats | Coag gene type | Phage type |
|---|---|---|---|---|---|---|---|---|
| Persistent carriers | ||||||||
| 53 | 44 | 09-03-92 | DD | 1 | DDD | 4 | 9C | 641 |
| 53 | 45 | 31-08-92 | FB | 1 | AAA | 7 | 12A | 793 |
| 53 | 47 | 01-11-93 | DD | 1 | GGG | 10 | 10F | 693 |
| 53 | 48 | 15-08-94 | DD | 1 | GGG | 9 | 10F | 542 |
| 84 | 54 | 09-03-92 | AA | 1 | JIH | 8 | 15I | 692 |
| 84 | 55 | 18-04-93 | AA | 1 | JIH | 7 | 15I | 692 |
| 84 | 56 | 01-11-93 | ED | 1 | BBB | 5 | 14B | 811 |
| 84 | 57 | 15-08-94 | BB | 2 | KJI | 9 | 9C | 292 |
| 126 | 58 | 19-10-92 | BB | 2 | LKC | 8 | 11N | 793 |
| 126 | 59 | 18-04-93 | ED | 1 | BBB | 7 | 14B | 812 |
| 126 | 60 | 15-08-94 | ED | 1 | BBB | 7 | 14B | 811 |
| Intermittent carriers | ||||||||
| 85 | 61 | 24-02-92 | AA | 1 | JIH | 7 | 15I | 311 |
| 85 | 62 | 09-03-92 | ED | 1 | BBB | 8 | 14B | 812 |
| 85 | 63 | 06-01-93 | ED | 1 | BBB | 8 | 14B | 232 |
| 85 | 64 | 15-08-94 | AA | 1 | JIH | 9 | 15I | 362 |
| 2 | 69 | 25-06-92 | ED | 1 | BBB | 9 | 14B | 813 |
| 2 | 70 | 19-10-92 | ED | 1 | BBB | 9 | 14B | 691 |
| 2 | 71 | 18-04-93 | AB | 1 | AAA | 7 | 12A | 793 |
| 2 | 72 | 14-02-94 | ED | 1 | BBB | 9 | 14B | 811 |
| 9 | 73 | 25-06-92 | ED | 1 | BBB | 5 | 14B | 811 |
| 9 | 74 | 19-10-92 | BA | 1 | CCC | 7 | 9C | 292 |
| 9 | 75 | 01-11-93 | BA | 1 | CCC | 7 | 9C | 233 |
| 9 | 76 | 15-08-94 | GG | 3 | MLJ | 5 | —b | 191 |
| 32 | 79 | 20-01-92 | ED | 1 | BBB | 3 | 14B | 811 |
| 32 | 80 | 25-06-92 | ED | 1 | BBB | 3 | 14B | 811 |
| 32 | 81 | 15-08-94 | DD | 1 | NMK | 9 | 9J | 793 |
| 20 | 84 | 09-03-92 | AA | 1 | PNC | 8 | 10G | 562 |
| 20 | 85 | 27-04-92 | AA | 1 | CCC | 10 | 9C | 293 |
| 20 | 86 | 25-06-92 | AA | 1 | BBB | 10 | 14B | 811 |
| 20 | 87 | 06-01-93 | ED | 1 | AAA | 7 | 12A | 793 |
| 113 | 88 | 09-03-92 | BB | 2 | JFH | 7 | 10I | 692 |
| 113 | 89 | 19-10-92 | AA | 1 | BBB | 10 | 14B | 811 |
| 113 | 90 | 14-02-94 | ED | 1 | OOL | 9 | 9C | 631 |
Data on the carrier status and RAPD, protein A (protein A (protA), coag gene, and phage types are adapted from van Belkum et al. (17). The RAPD three-letter code summarizes the typing results for the primer used (first letter, primer AP-1; second letter, primer AP-7; third letter, primer ERIC-2). Numbers of protein A repeats were derived from an analysis of the so-called X region by PCR. Amplicons were digested with RsaI. The number of direct repeats in this region was determined after electrophoresis. Coag gene types were derived from a PCR-RFLP analysis of the coag gene and subsequent detection of the amplicon with AluI. Phage types are given in an abbreviated form according to standards applied in the Statens Seruminstitut, Copenhagen, Denmark.
—, no restriction site for the amplicon.