Table 1.

Reagents for PCR

PCR components	Volume (µl)
Nuclease-free water	10.70
10× reaction buffer with MgCl2 (1.5 mM)	2.00
dNTP mix (2.5 mM)	2.00
Primer FP (10 pmol/µl)	2.00
Primer RP (10 pmol/µl)	2.00
Taq DNA polymerase (5 U)	0.30
Template DNA (50 ng/µl)	1.00
Total volume	20.0

PCR=polymerase chain reaction, dNTP=deoxyribose nucleotide triphosphate, FP=Forward Primer, RP=Reverse Primer