Fig. 3.

Depletion of ZNF384 expression suppresses breast cancer metastasis. A ZNF384 expression in stable ZNF384-depleted cells (shZNF384) and control cells (shControl) determined by western blot. B The ability of cell proliferation of cells as in A determined by CCK-8. C The clonogenic potential of cells as in A determined by colony formation assays. D–F The ability of cell migration and invasion in cells as in A determined by wound-healing (D) and transwell (E–F) assays. G Tumor growth curves for xenograft mice injected with cells as in (A) at the indicated times. H Bioluminescent imaging of metastasis for xenograft mice after tail vein injection of cells as in A. I H&E staining or immunohistochemical staining in primary tumors or metastatic nodules in the lung harvested from mice bearing the indicated xenograft tumors. *P < 0.05