Fig. 7.

ZEB1 represses miR-485-5p expression in a ZNF384–ZEB1 feedback loop. A A potential ZEB1-binding site located in the miR-485 promoter region. B The interaction between ZEB1 and miR-485 promoter region in MDA-MB-231 cells verified by ChIP analysis. C The effect of ZEB1 expression on miR-485 promoter activity determined by dual-luciferase reporter analysis in 293FT cells. A series of fragments containing the predicted ZEB1 binding site is fused upstream of Luc gene. D The expression of miR-485-5p levels in ZEB1-depleted MDA-MB-231 cells or ZEB1-overexpressed T47D cells, as well as control cells, determined by RT-qPCR. E The expression of miR-485-5p levels in ZNF384-depleted MDA-MB-231 cells or ZNF384-overexpressed T47D cells, as well as control cells, determined by RT-qPCR. F Schematic illustration of ZNF384-miR-485-5p feedback loop in regulating breast cancer EMT and metastasis. *P < 0.05