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. 2022 Sep 13;10:147. doi: 10.1186/s40168-022-01335-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Variations in gut archaeome across healthy individuals. a Geographical distribution of the studied Chinese populations. Yunnan Province was the sampling region. Urban individuals were recruited and sampled from the Kunming City (the provincial capital of Yunnan Province), consisting of individuals from 6 ethnicities (Han, Zang, Miao, Bai, Dai, and Hani). Rural populations corresponding to each ethnicity were individually recruited and sampled at each rural region circumferential of Kunming. b Number of subjects recruited in this study (n = 792). c An overview of study design and data collection regime, including archaeome and bacteriome profiling, metadata questionnaire investigation, diet record (food frequency questionnaire) investigation, and blood biochemical measurements. d Variations in gut archaeome composition at the order level across all study subjects, plotted according to the ranking abundance of archaeal orders. e Order-level gut archaeome compositions plotted according to ethnicity (Han, Zang, Miao, Bai, Dai, and Hani) and residency (rural versus urban)