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. 2022 Aug 28;8(9):e10365. doi: 10.1016/j.heliyon.2022.e10365

Figure 2.

Figure 2

Morphological representation and beating frequency variation of cardiac organoids during doxorubicin exposure. Human iPSCs were differentiated into cardiac organoids according to the established protocol and incubated on day 24 of cultivation for 24 h, 48 h and 72 h with 1 μM, 3 μM or 10 μM doxorubicin (DOX). (A) Representative Phase contrast images during DOX treatment. Scale bar: 100 μm (B) Beating frequency of completely contracting cardiac organoids during DOX treatment. Results were replicated in 3 independent differentiation experiments. Mean ± SD. One Way ANOVA with Tukey's post test with ∗∗∗∗p < 0.0001. DOX: Doxorubicin.