Table 6.

Thermocycler PCR program.

PCR Cycling Conditions
Steps	Temperature	Time	Cycles
Initial denaturation	95 °C	3 min	1
Denaturation	94 °C	15 s	30–35 cycles
Annealing	56–60 °C	30 s
Extension	72 °C	30 s
Final extension	72 °C	10 min	1
Hold	4 °C	∞