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. Author manuscript; available in PMC: 2022 Sep 13.
Published in final edited form as: Glia. 2020 Dec 12;69(4):1022–1036. doi: 10.1002/glia.23946

FIGURE 5.

FIGURE 5

OEC-CM suppresses multiple transcripts associated with neurotoxic astrocyte reactivity in LPS-treated astrocytes. Heat map illustrating results of Q-RT-PCR (Data S3) to detect neurotoxic astrocyte transcripts in primary astrocytes treated with LPS (A); LPS plus WT OEC-CM (B); LPS plus CryAB−/−OEC-CM (C); or IP-CryAB for 24 hr (D). Compared with LPS, OEC-CM (B) significantly suppressed 9 transcripts, whereas CryAB−/−OEC-CM (C) significantly suppressed 4. (D) IP-CryAB suppressed 6 of the 9 transcripts suppressed by OEC-CM. In addition, CryAB−/−OEC-CM + LPS caused a significant increase in 4 transcripts, while IP-CryAB caused a significant increase in one. Finally, one transcript, C3, was significantly suppressed by both OEC-CM treatments (CryAB−/− or WT) but was elevated by IP-CryAB treatment. Q-RT-PCR experiments were analyzed by one-way ANOVA followed by Dunnett’s multiple post hoc test (n = 3; arrows p ≤ .05)